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New species of green microalga identified in São Paulo

A group led by researchers affiliated with the Phycology Laboratory at the Federal University of São Carlos (UFSCar) in the state of São Paulo, Brazil, have discovered a new species of green microalga in a reservoir located in the northwest of the state..

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A group led by researchers affiliated with the Phycology Laboratory at the Federal University of São Carlos (UFSCar) in the state of São Paulo, Brazil, have discovered a new species of green microalga in a reservoir located in the northwest of the state. As a result of the discovery, microalgae of the genus Nephrocytium have been moved to an order belonging to a different taxonomic class and phylogenetically reclassified (placed in a new family).

Credit: CCMA/UFSCar

A group led by researchers affiliated with the Phycology Laboratory at the Federal University of São Carlos (UFSCar) in the state of São Paulo, Brazil, have discovered a new species of green microalga in a reservoir located in the northwest of the state. As a result of the discovery, microalgae of the genus Nephrocytium have been moved to an order belonging to a different taxonomic class and phylogenetically reclassified (placed in a new family).

Green microalgae are found in freshwater and in the sea (where they are known as phytoplankton) and produce more than 80% of the oxygen present in the atmosphere. They are considered primary producers and hence the base of the food chain in aquatic environments. In addition, they have significant potential for commercial applications, such as biodiesel production.

The new species was found in a fishing pond called Muritiba, fed by a spring near the town of Tupã. The species was isolated in 2014 and is now part of UFSCar’s Freshwater Microalgae Culture Collection (CCMA). Begun in 1977 by Armando Augusto Henriques Vieira early in his career as a professor in the Department of Botany, CCMA is one of the world’s largest and oldest collections of freshwater microalgae, with some 800 strains deposited and conserved for researchers to analyze and potentially commercialize.

The new species was named Nephrocytium vieirae as a tribute to Vieira, who has retired and is now a senior professor at the university. The research that identified it was part of several projects supported by FAPESP (11/50054-412/19520-113/17457-313/18083-0 and 16/07089-5). 

The study involved samples collected at more than 300 sites including reservoirs, marches, bogs and lakes in all 22 hydrographic regions of the state. After collection by various scientists under Vieira’s supervision, the material was isolated in the laboratory and identified on the basis of morphology. During this process, a particular alga was isolated and identified as a morphotype similar to Nephrocytium. In taxonomy, a morphotype is an individual or set of organisms distinguished within the same species by having a certain physical structure.

Inessa Lacativa Bagatini, now a professor in UFSCar’s Department of Botany, was then a postdoctoral fellow under Vieira. After cleansing the specimen of contaminants such as fungi and bacteria, Bagatini found a molecular marker by polymerase chain reaction (PCR) sequencing. The marker placed the strain in the genus Nephrocytium, but pointed to the possibility that a new species had been found.

Thaís Garcia da Silva, first author of an article published on the research, was then studying different algal morphotypes for her doctorate. She sequenced other markers in the specimens and analyzed their morphology under an optical microscope. She then submitted them to transmission electron microscopy, cleaving cells to study the organelles, and scanning electron microscopy, analyzing the cell surface. This part of the research was assisted by Naiara Carolina Pereira Archanjo, also a researcher in UFSCar’s Department of Botany and a PhD candidate at the time.

Silva and Bagatini also collaborated with Lenka Štenclová, a researcher at the University of South Bohemia in the Czech Republic, to obtain molecular markers of a Nephrocytium species not available from CCMA at UFSCar. Integration of all this data confirmed that the DNA sequence obtained for the isolated strain was different from those of other species described in the literature. “Microalgae can have very similar morphology in various situations so that molecular markers must be used to decide whether a new species has been discovered, as was the case here,” Silva explained.

The next step was the mapping of a phylogenetic tree for Nephrocytium vieirae. Phylogeny traces the evolutionary history of a species, organizing beings into hierarchical taxonomic categories such as kingdom, phylum, class, order, family, genus and species. A phylogenetic tree depicts the lines of evolutionary descent from a common ancestor, rather as a genealogical tree does for human families.

“When we were constructing the phylogenetic tree, we confirmed the suggestion in recent research that the genus belonged to a different class,” Silva said. In this stage of the study, the microalga in question was compared with microalgae in all families belonging to the order Sphaeropleales, to which it is allocated, and the researchers conducted a review of the scientific literature in which occurrences of the genus Nephrocytium were mentioned.

Phylogenetic reclassification

In the course of reclassifying the new species, the researchers noted several traits that would not let them keep microalgae of the genus Nephrocytium in the class Trebouxiophyceae, order Chlorellales and family Oocystaceae, as per the scientific literature. Based on the data they obtained in their various analyses, they proposed the creation of a new family, Nephrocytiaceae, belonging to the class Chlorophyceae and order Sphaeropleales.

The article with their findings is published in Taxon, the journal of the International Association for Plant Taxonomy (IAPT), and signed by Silva, Bagatini, Štenclová and Archanjo.

As detailed in the article, microalgae of the family Nephrocytiaceae, including the newly discovered species N. vieirae, can be distinguished from others in the order Sphaeropleales by a combination of morphological traits, smooth cell surfaces, asexual reproduction via autospores, differences in ultrastructure (cell architecture visible at higher magnifications than found on a standard optical microscope), and molecular data. These characteristics corroborated their phylogenetic reclassification and placement in a new family.

Other reports of the new species

Publication of the results enables other researchers in Brazil and elsewhere to use the molecular markers to find out whether specimens can be classified as N. vieirae. “The morphology of N. lunatum closely resembles that of the recent discovery in Tupã. Anyone who has collected a Nephrocytium and not done a molecular phylogenetic analysis is likely to think it’s N. lunatum. Publication of our findings showing that we now have this marker could enable others to identify microalgae of the new species in other countries where massive parallel sequencing is used,” Bagatini said.

Massive parallel sequencing is widely deployed in research on microalgae. It entails simultaneous sequencing of many samples without observing morphology and comparing the results with sequences stored in a database. “If no matches with other organisms are found, the specimen is placed in the closest genus or taxonomic group,” Bagatini explained. The alternative is morphological and molecular analysis of individual organisms.

Correct classification is essential not only for researchers to be able to continue their studies but also for the development of industrial and biotech applications in general. “If you’re studying a species to develop a cancer drug, for example, and you want to look at others that maybe could produce more of the compound in question, you’ll look for a species that’s phylogenetically close,” Bagatini said, adding that incorrect taxonomic classification can lead to selection of organisms that lack the desired characteristics.

UFSCar’s microalgae collection

The new microalga joins a collection formed from a project that started with the sea. In 1970, Vieira was an undergraduate studying biology at the University of São Paulo (USP) and embarking on an internship at the Oceanographic Institute (IO-USP), where he would organize a marine phytoplankton collection. There he learned how to isolate marine microalgae and managed to mount a collection of some 100 strains. He worked at IO-USP until 1977, when he moved to UFSCar to focus on freshwater microalgae, whose study he decided to prioritize after attending a lecture on these organisms while still an undergraduate. UFSCar’s collection began with specimens collected in the vicinity of the university, but microalgae were soon being collected in other areas of the state thanks to funding won by Vieira. 

“We improved the quality of the collection as time passed, and some four years ago we implemented a cryogenic system to conserve microalgae in liquid nitrogen,” he said.

UFSCar’s collection has been used for research on biodiesel and to test swimming pool water treatment systems, as well as in research projects at several institutions around Brazil conducted by master’s and PhD candidates, and even by high school students. “Demand was low for a long time but has risen in the last few years because of an increase in potential uses,” Vieira said.

After retiring, he handed over to Bagatini as curator of the collection. “I’ve continued to help, and I plan to return and resume my support when the pandemic permits. There’s still plenty of material in the collection that needs to be studied,” he said.

Asked how he felt about having a species named after him, he said, “I’m flattered and grateful.” Because of this homage, his name does not appear as an author of the article, even though he supervised the team. “I was lucky to have such good students. They’re now fully qualified professionals working in Brazil and abroad,” he noted.

About São Paulo Research Foundation (FAPESP)

The São Paulo Research Foundation (FAPESP) is a public institution with the mission of supporting scientific research in all fields of knowledge by awarding scholarships, fellowships and grants to investigators linked with higher education and research institutions in the State of São Paulo, Brazil. FAPESP is aware that the very best research can only be done by working with the best researchers internationally. Therefore, it has established partnerships with funding agencies, higher education, private companies, and research organizations in other countries known for the quality of their research and has been encouraging scientists funded by its grants to further develop their international collaboration. You can learn more about FAPESP at www.fapesp.br/en and visit FAPESP news agency at www.agencia.fapesp.br/en to keep updated with the latest scientific breakthroughs FAPESP helps achieve through its many programs, awards and research centers. You may also subscribe to FAPESP news agency at http://agencia.fapesp.br/subscribe.


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The Coming Of The Police State In America

The Coming Of The Police State In America

Authored by Jeffrey Tucker via The Epoch Times,

The National Guard and the State Police are now…

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The Coming Of The Police State In America

Authored by Jeffrey Tucker via The Epoch Times,

The National Guard and the State Police are now patrolling the New York City subway system in an attempt to do something about the explosion of crime. As part of this, there are bag checks and new surveillance of all passengers. No legislation, no debate, just an edict from the mayor.

Many citizens who rely on this system for transportation might welcome this. It’s a city of strict gun control, and no one knows for sure if they have the right to defend themselves. Merchants have been harassed and even arrested for trying to stop looting and pillaging in their own shops.

The message has been sent: Only the police can do this job. Whether they do it or not is another matter.

Things on the subway system have gotten crazy. If you know it well, you can manage to travel safely, but visitors to the city who take the wrong train at the wrong time are taking grave risks.

In actual fact, it’s guaranteed that this will only end in confiscating knives and other things that people carry in order to protect themselves while leaving the actual criminals even more free to prey on citizens.

The law-abiding will suffer and the criminals will grow more numerous. It will not end well.

When you step back from the details, what we have is the dawning of a genuine police state in the United States. It only starts in New York City. Where is the Guard going to be deployed next? Anywhere is possible.

If the crime is bad enough, citizens will welcome it. It must have been this way in most times and places that when the police state arrives, the people cheer.

We will all have our own stories of how this came to be. Some might begin with the passage of the Patriot Act and the establishment of the Department of Homeland Security in 2001. Some will focus on gun control and the taking away of citizens’ rights to defend themselves.

My own version of events is closer in time. It began four years ago this month with lockdowns. That’s what shattered the capacity of civil society to function in the United States. Everything that has happened since follows like one domino tumbling after another.

It goes like this:

1) lockdown,

2) loss of moral compass and spreading of loneliness and nihilism,

3) rioting resulting from citizen frustration, 4) police absent because of ideological hectoring,

5) a rise in uncontrolled immigration/refugees,

6) an epidemic of ill health from substance abuse and otherwise,

7) businesses flee the city

8) cities fall into decay, and that results in

9) more surveillance and police state.

The 10th stage is the sacking of liberty and civilization itself.

It doesn’t fall out this way at every point in history, but this seems like a solid outline of what happened in this case. Four years is a very short period of time to see all of this unfold. But it is a fact that New York City was more-or-less civilized only four years ago. No one could have predicted that it would come to this so quickly.

But once the lockdowns happened, all bets were off. Here we had a policy that most directly trampled on all freedoms that we had taken for granted. Schools, businesses, and churches were slammed shut, with various levels of enforcement. The entire workforce was divided between essential and nonessential, and there was widespread confusion about who precisely was in charge of designating and enforcing this.

It felt like martial law at the time, as if all normal civilian law had been displaced by something else. That something had to do with public health, but there was clearly more going on, because suddenly our social media posts were censored and we were being asked to do things that made no sense, such as mask up for a virus that evaded mask protection and walk in only one direction in grocery aisles.

Vast amounts of the white-collar workforce stayed home—and their kids, too—until it became too much to bear. The city became a ghost town. Most U.S. cities were the same.

As the months of disaster rolled on, the captives were let out of their houses for the summer in order to protest racism but no other reason. As a way of excusing this, the same public health authorities said that racism was a virus as bad as COVID-19, so therefore it was permitted.

The protests had turned to riots in many cities, and the police were being defunded and discouraged to do anything about the problem. Citizens watched in horror as downtowns burned and drug-crazed freaks took over whole sections of cities. It was like every standard of decency had been zapped out of an entire swath of the population.

Meanwhile, large checks were arriving in people’s bank accounts, defying every normal economic expectation. How could people not be working and get their bank accounts more flush with cash than ever? There was a new law that didn’t even require that people pay rent. How weird was that? Even student loans didn’t need to be paid.

By the fall, recess from lockdown was over and everyone was told to go home again. But this time they had a job to do: They were supposed to vote. Not at the polling places, because going there would only spread germs, or so the media said. When the voting results finally came in, it was the absentee ballots that swung the election in favor of the opposition party that actually wanted more lockdowns and eventually pushed vaccine mandates on the whole population.

The new party in control took note of the large population movements out of cities and states that they controlled. This would have a large effect on voting patterns in the future. But they had a plan. They would open the borders to millions of people in the guise of caring for refugees. These new warm bodies would become voters in time and certainly count on the census when it came time to reapportion political power.

Meanwhile, the native population had begun to swim in ill health from substance abuse, widespread depression, and demoralization, plus vaccine injury. This increased dependency on the very institutions that had caused the problem in the first place: the medical/scientific establishment.

The rise of crime drove the small businesses out of the city. They had barely survived the lockdowns, but they certainly could not survive the crime epidemic. This undermined the tax base of the city and allowed the criminals to take further control.

The same cities became sanctuaries for the waves of migrants sacking the country, and partisan mayors actually used tax dollars to house these invaders in high-end hotels in the name of having compassion for the stranger. Citizens were pushed out to make way for rampaging migrant hordes, as incredible as this seems.

But with that, of course, crime rose ever further, inciting citizen anger and providing a pretext to bring in the police state in the form of the National Guard, now tasked with cracking down on crime in the transportation system.

What’s the next step? It’s probably already here: mass surveillance and censorship, plus ever-expanding police power. This will be accompanied by further population movements, as those with the means to do so flee the city and even the country and leave it for everyone else to suffer.

As I tell the story, all of this seems inevitable. It is not. It could have been stopped at any point. A wise and prudent political leadership could have admitted the error from the beginning and called on the country to rediscover freedom, decency, and the difference between right and wrong. But ego and pride stopped that from happening, and we are left with the consequences.

The government grows ever bigger and civil society ever less capable of managing itself in large urban centers. Disaster is unfolding in real time, mitigated only by a rising stock market and a financial system that has yet to fall apart completely.

Are we at the middle stages of total collapse, or at the point where the population and people in leadership positions wise up and decide to put an end to the downward slide? It’s hard to know. But this much we do know: There is a growing pocket of resistance out there that is fed up and refuses to sit by and watch this great country be sacked and taken over by everything it was set up to prevent.

Tyler Durden Sat, 03/09/2024 - 16:20

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Another beloved brewery files Chapter 11 bankruptcy

The beer industry has been devastated by covid, changing tastes, and maybe fallout from the Bud Light scandal.

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Before the covid pandemic, craft beer was having a moment. Most cities had multiple breweries and taprooms with some having so many that people put together the brewery version of a pub crawl.

It was a period where beer snobbery ruled the day and it was not uncommon to hear bar patrons discuss the makeup of the beer the beer they were drinking. This boom period always seemed destined for failure, or at least a retraction as many markets seemed to have more craft breweries than they could support.

Related: Fast-food chain closes more stores after Chapter 11 bankruptcy

The pandemic, however, hastened that downfall. Many of these local and regional craft breweries counted on in-person sales to drive their business. 

And while many had local and regional distribution, selling through a third party comes with much lower margins. Direct sales drove their business and the pandemic forced many breweries to shut down their taprooms during the period where social distancing rules were in effect.

During those months the breweries still had rent and employees to pay while little money was coming in. That led to a number of popular beermakers including San Francisco's nationally-known Anchor Brewing as well as many regional favorites including Chicago’s Metropolitan Brewing, New Jersey’s Flying Fish, Denver’s Joyride Brewing, Tampa’s Zydeco Brew Werks, and Cleveland’s Terrestrial Brewing filing bankruptcy.

Some of these brands hope to survive, but others, including Anchor Brewing, fell into Chapter 7 liquidation. Now, another domino has fallen as a popular regional brewery has filed for Chapter 11 bankruptcy protection.

Overall beer sales have fallen.

Image source: Shutterstock

Covid is not the only reason for brewery bankruptcies

While covid deserves some of the blame for brewery failures, it's not the only reason why so many have filed for bankruptcy protection. Overall beer sales have fallen driven by younger people embracing non-alcoholic cocktails, and the rise in popularity of non-beer alcoholic offerings,

Beer sales have fallen to their lowest levels since 1999 and some industry analysts

"Sales declined by more than 5% in the first nine months of the year, dragged down not only by the backlash and boycotts against Anheuser-Busch-owned Bud Light but the changing habits of younger drinkers," according to data from Beer Marketer’s Insights published by the New York Post.

Bud Light parent Anheuser Busch InBev (BUD) faced massive boycotts after it partnered with transgender social media influencer Dylan Mulvaney. It was a very small partnership but it led to a right-wing backlash spurred on by Kid Rock, who posted a video on social media where he chastised the company before shooting up cases of Bud Light with an automatic weapon.

Another brewery files Chapter 11 bankruptcy

Gizmo Brew Works, which does business under the name Roth Brewing Company LLC, filed for Chapter 11 bankruptcy protection on March 8. In its filing, the company checked the box that indicates that its debts are less than $7.5 million and it chooses to proceed under Subchapter V of Chapter 11. 

"Both small business and subchapter V cases are treated differently than a traditional chapter 11 case primarily due to accelerated deadlines and the speed with which the plan is confirmed," USCourts.gov explained. 

Roth Brewing/Gizmo Brew Works shared that it has 50-99 creditors and assets $100,000 and $500,000. The filing noted that the company does expect to have funds available for unsecured creditors. 

The popular brewery operates three taprooms and sells its beer to go at those locations.

"Join us at Gizmo Brew Works Craft Brewery and Taprooms located in Raleigh, Durham, and Chapel Hill, North Carolina. Find us for entertainment, live music, food trucks, beer specials, and most importantly, great-tasting craft beer by Gizmo Brew Works," the company shared on its website.

The company estimates that it has between $1 and $10 million in liabilities (a broad range as the bankruptcy form does not provide a space to be more specific).

Gizmo Brew Works/Roth Brewing did not share a reorganization or funding plan in its bankruptcy filing. An email request for comment sent through the company's contact page was not immediately returned.

 

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Revving up tourism: Formula One and other big events look set to drive growth in the hospitality industry

With big events drawing a growing share of of tourism dollars, F1 offers a potential glimpse of the travel industry’s future.

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Sergio Perez of Oracle Red Bull Racing, right, and Charles Leclerc of the Scuderia Ferrari team compete in the Las Vegas Grand Prix on Nov. 19, 2023. Tayfun Coskun/Anadolu via Getty Images

In late 2023, I embarked on my first Formula One race experience, attending the first-ever Las Vegas Grand Prix. I had never been to an F1 race; my interest was sparked during the pandemic, largely through the Netflix series “Formula 1: Drive to Survive.”

But I wasn’t just attending as a fan. As the inaugural chair of the University of Florida’s department of tourism, hospitality and event management, I saw this as an opportunity. Big events and festivals represent a growing share of the tourism market – as an educator, I want to prepare future leaders to manage them.

And what better place to learn how to do that than in the stands of the Las Vegas Grand Prix?

A smiling professor is illuminated by bright lights in a nighttime photo taken at a Formula 1 event in Nevada.
The author at the Las Vegas Grand Prix. Katherine Fu

The future of tourism is in events and experiences

Tourism is fun, but it’s also big business: In the U.S. alone, it’s a US$2.6 trillion industry employing 15 million people. And with travelers increasingly planning their trips around events rather than places, both industry leaders and academics are paying attention.

Event tourism is also key to many cities’ economic development strategies – think Chicago and its annual Lollapalooza music festival, which has been hosted in Grant Park since 2005. In 2023, Lollapalooza generated an estimated $422 million for the local economy and drew record-breaking crowds to the city’s hotels.

That’s why when Formula One announced it would be making a 10-year commitment to host races in Las Vegas, the region’s tourism agency was eager to spread the news. The 2023 grand prix eventually generated $100 million in tax revenue, the head of that agency later announced.

Why Formula One?

Formula One offers a prime example of the economic importance of event tourism. In 2022, Formula One generated about $2.6 billion in total revenues, according to the latest full-year data from its parent company. That’s up 20% from 2021 and 27% from 2019, the last pre-COVID year. A record 5.7 million fans attended Formula One races in 2022, up 36% from 2019.

This surge in interest can be attributed to expanded broadcasting rights, sponsorship deals and a growing global fan base. And, of course, the in-person events make a lot of money – the cheapest tickets to the Las Vegas Grand Prix were $500.

Two brightly colored race cars are seen speeding down a track in a blur.
Turn 1 at the first Las Vegas Grand Prix. Rachel Fu, CC BY

That’s why I think of Formula One as more than just a pastime: It’s emblematic of a major shift in the tourism industry that offers substantial job opportunities. And it takes more than drivers and pit crews to make Formula One run – it takes a diverse range of professionals in fields such as event management, marketing, engineering and beyond.

This rapid industry growth indicates an opportune moment for universities to adapt their hospitality and business curricula and prepare students for careers in this profitable field.

How hospitality and business programs should prepare students

To align with the evolving landscape of mega-events like Formula One races, hospitality schools should, I believe, integrate specialized training in event management, luxury hospitality and international business. Courses focusing on large-scale event planning, VIP client management and cross-cultural communication are essential.

Another area for curriculum enhancement is sustainability and innovation in hospitality. Formula One, like many other companies, has increased its emphasis on environmental responsibility in recent years. While some critics have been skeptical of this push, I think it makes sense. After all, the event tourism industry both contributes to climate change and is threatened by it. So, programs may consider incorporating courses in sustainable event management, eco-friendly hospitality practices and innovations in sustainable event and tourism.

Additionally, business programs may consider emphasizing strategic marketing, brand management and digital media strategies for F1 and for the larger event-tourism space. As both continue to evolve, understanding how to leverage digital platforms, engage global audiences and create compelling brand narratives becomes increasingly important.

Beyond hospitality and business, other disciplines such as material sciences, engineering and data analytics can also integrate F1 into their curricula. Given the younger generation’s growing interest in motor sports, embedding F1 case studies and projects in these programs can enhance student engagement and provide practical applications of theoretical concepts.

Racing into the future: Formula One today and tomorrow

F1 has boosted its outreach to younger audiences in recent years and has also acted to strengthen its presence in the U.S., a market with major potential for the sport. The 2023 Las Vegas race was a strategic move in this direction. These decisions, along with the continued growth of the sport’s fan base and sponsorship deals, underscore F1’s economic significance and future potential.

Looking ahead in 2024, Formula One seems ripe for further expansion. New races, continued advancements in broadcasting technology and evolving sponsorship models are expected to drive revenue growth. And Season 6 of “Drive to Survive” will be released on Feb. 23, 2024. We already know that was effective marketing – after all, it inspired me to check out the Las Vegas Grand Prix.

I’m more sure than ever that big events like this will play a major role in the future of tourism – a message I’ll be imparting to my students. And in my free time, I’m planning to enhance my quality of life in 2024 by synchronizing my vacations with the F1 calendar. After all, nothing says “relaxing getaway” quite like the roar of engines and excitement of the racetrack.

Rachel J.C. Fu does not work for, consult, own shares in or receive funding from any company or organization that would benefit from this article, and has disclosed no relevant affiliations beyond their academic appointment.

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