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Lineage tracing of direct astrocyte-to-neuron conversion for brain repair

Lineage tracing of direct astrocyte-to-neuron conversion for brain repair

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Credit: Jinan University

Regeneration of functional new neurons to repair injured human brain is a long-term unsolved problem up till today. The lack of neuroregeneration is one of the major reasons why so many brain disorders such as stroke and Alzheimer’s disease do not have a cure yet. A research team led by Prof. Gong Chen at Jinan University (Guangzhou, China) published a work on October 9th in Neural Regeneration Research, providing unambiguous data that brain internal astrocytes are directly converted into neurons through lineage tracing studies. Using brain internal astrocytes, a type of supporting cells to neurons, to directly convert into new neurons is an innovative brain repair technology that may benefit millions of patients worldwide.

Human brain has two major types of cells, neurons and glial cells. Neurons cannot divide and therefore don’t have self-regeneration capability, but glial cells can divide and self-regenerate. Prof. Chen’s team has previously demonstrated through a series of works that brain internal glial cells can be directly converted into neurons through NeuroD1-based gene therapy. Many other labs have also confirmed such direct glia-to-neuron conversion in the brain, spinal cord, and retina. However, some people, both inside and outside the regenerative medicine field, still have some benefit of the doubt on direct glia-to-neuron conversion and demand more evidence to support such ground-breaking discoveries.

In this study, Chen’s team employs transgenic reporter mice that can faithfully trace astrocytic lineage to unambiguously demonstrate that astrocytes can be converted directly into neurons in adult mouse brain. “We crossed Aldh1l1-CreERT2 mice with Ai14 mice and administered tamoxifen to induce Cre-mediated recombination so that many astrocytes will be lineage-traced permanently by red fluorescent protein tdTomato”, explained by the first author and a postdoctoral fellow Dr. Zongqin Xiang. “Then, we injected AAV expressing neural transcription factor NeuroD1 under astrocytic promoter GFAP into the mouse cortex, and detected clear expression of NeuroD1 in the tdTomato-labeled astrocytes at 7 days post viral infection”, added by the co-first author and a PhD student Mr. Liang Xu. “Most excitingly, at 135 days post NeuroD1 infection (experiments delayed by COVID-19), we observed that many NeuroD1-expressing tdTomato-labeled astrocytes had now been converted into NeuN-positive neurons with typical neuronal morphology. This experiment provides unambiguous evidence on in vivo astrocyte-to-neuron conversion”, concluded by Prof. Gong Chen.

Besides AAV viral system, Chen’s team employed another viral system, the retroviral system, to further demonstrate such direct glia-to-neuron conversion process. “While AAV has the advantage of low immunogenicity and relatively safe as a gene therapy vector for the treatment of neurological disorders, its capability to infect both neurons and glial cells may cause confusion if AAV dosing and promoter are not used properly,” said Prof. Wenliang Lei, a co-corresponding author of the work. “We therefore employed retroviral vectors to express NeuroD1 exclusively in dividing glial cells, and we were able to further confirm that the newly generated neurons were directly converted from dividing glial cells”, added Prof. Lei.

With two lines of unambiguous data in hand, Chen’s team went on to address some confusions in the field caused by some improperly designed experiments and high dosing of AAV used by some labs. “A recent work challenged the field of in vivo glia-to-neuron conversion based on one set of experiments using high dose of AAV that produced artifacts in the mouse cortex,” said Prof. Wen Li, another co-corresponding author of the work. “We demonstrated in this work that when an appropriate dosage of AAV was used, the artifacts of so-called leakage can be minimized if not completely avoided”, added Prof. Li.

“For newcomers entering into this exciting in vivo glia-to-neuron conversion field, we highly recommend all investigators using different viral systems at different dosing, and performing both in vitro and in vivo studies to prove or disprove any hypothesis,” Prof. Chen provided insightful comments as a leader of this emerging new field. “With the addition of this indisputable evidence from lineage tracing studies, we believe that this exciting in vivo glia-to-neuron conversion technology will provide an unprecedented opportunity to repair damaged brain with internal glial cells”, Prof. Chen concluded with unwavering confidence.

###

The authors contributed to this work include Dr. Zongqin Xiang, Mr. Liang Xu, Ms. Minhui Liu, Dr. Qingsong Wang, Dr. Wen Li, Dr. Wenliang Lei, and Dr. Gong Chen, all at Jinan University.

Article link:

Xiang Z, Xu L, Liu M, Wang Q, Li W, Lei W, Chen G. Lineage tracing of direct astrocyte-to-neuron conversion in the mouse cortex. Neural Regen Res 2021;16:750-6.
Available from: http://www.nrronline.org/text.asp?2021/16/4/750/295925

Media Contact
Qingsong Wang
qingsongwang@jnu.edu.cn

Original Source

https://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=4;spage=750;epage=756;aulast=Xiang

Related Journal Article

http://dx.doi.org/10.4103/1673-5374.295925

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How Fanatics Took Over The World

How Fanatics Took Over The World

Authored by Jeffrey Tucker via DailyReckoning.com,

Early in the pandemic, I had been furiously writing articles about lockdowns. My phone rang with a call from a man named Dr. Rajeev Venkayya. He is the head.

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How Fanatics Took Over The World

Authored by Jeffrey Tucker via DailyReckoning.com,

Early in the pandemic, I had been furiously writing articles about lockdowns. My phone rang with a call from a man named Dr. Rajeev Venkayya. He is the head of a vaccine company but introduced himself as former head of pandemic policy for the Gates Foundation.

Now I was listening.

I did not know it then, but I’ve since learned from Michael Lewis’s (mostly terrible) book The Premonition that Venkayya was, in fact, the founding father of lockdowns. While working for George W. Bush’s White House in 2005, he headed a bioterrorism study group. From his perch of influence – serving an apocalyptic president — he was the driving force for a dramatic change in U.S. policy during pandemics.

He literally unleashed hell.

That was 15 years ago. At the time, I wrote about the changes I was witnessing, worrying that new White House guidelines (never voted on by Congress) allowed the government to put Americans in quarantine while closing their schools, businesses, and churches shuttered, all in the name of disease containment.

I never believed it would happen in real life; surely there would be public revolt. Little did I know, we were in for a wild ride…

The Man Who Lit the Match

Last year, Venkayya and I had a 30-minute conversation; actually, it was mostly an argument. He was convinced that lockdown was the only way to deal with a virus. I countered that it was wrecking rights, destroying businesses, and disturbing public health. He said it was our only choice because we had to wait for a vaccine. I spoke about natural immunity, which he called brutal. So on it went.

The more interesting question I had at the time was why this certified Big Shot was wasting his time trying to convince a poor scribbler like me. What possible reason could there be?

The answer, I now realized, is that from February to April 2020, I was one of the few people (along with a team of researchers) who openly and aggressively opposed what was happening.

There was a hint of insecurity and even fear in Venkayya’s voice. He saw the awesome thing he had unleashed all over the world and was anxious to tamp down any hint of opposition. He was trying to silence me. He and others were determined to crush all dissent.

This is how it has been for the better part of the last 15 months, with social media and YouTube deleting videos that dissent from lockdowns. It’s been censorship from the beginning.

For all the problems with Lewis’s book, and there are plenty, he gets this whole backstory right. Bush came to his bioterrorism people and demanded some huge plan to deal with some imagined calamity. When Bush saw the conventional plan — make a threat assessment, distribute therapeutics, work toward a vaccine — he was furious.

“This is bulls**t,” the president yelled.

“We need a whole-of-society plan. What are you going to do about foreign borders? And travel? And commerce?”

Hey, if the president wants a plan, he’ll get a plan.

“We want to use all instruments of national power to confront this threat,” Venkayya reports having told colleagues.

“We were going to invent pandemic planning.”

This was October 2005, the birth of the lockdown idea.

Dr. Venkayya began to fish around for people who could come up with the domestic equivalent of Operation Desert Storm to deal with a new virus. He found no serious epidemiologists to help. They were too smart to buy into it. He eventually bumped into the real lockdown innovator working at Sandia National Laboratories in New Mexico.

Cranks, Computers, and Cooties

His name was Robert Glass, a computer scientist with no medical training, much less knowledge, about viruses. Glass, in turn, was inspired by a science fair project that his 14-year-old daughter was working on.

She theorized (like the cooties game from grade school) that if school kids could space themselves out more or even not be at school at all, they would stop making each other sick. Glass ran with the idea and banged out a model of disease control based on stay-at-home orders, travel restrictions, business closures, and forced human separation.

Crazy right? No one in public health agreed with him but like any classic crank, this convinced Glass even more. I asked myself, “Why didn’t these epidemiologists figure it out?” They didn’t figure it out because they didn’t have tools that were focused on the problem. They had tools to understand the movement of infectious diseases without the purpose of trying to stop them.

Genius, right? Glass imagined himself to be smarter than 100 years of experience in public health. One guy with a fancy computer would solve everything! Well, he managed to convince some people, including another person hanging around the White House named Carter Mecher, who became Glass’s apostle.

Please consider the following quotation from Dr. Mecher in Lewis’s book: “If you got everyone and locked each of them in their own room and didn’t let them talk to anyone, you would not have any disease.”

At last, an intellectual has a plan to abolish disease — and human life as we know it too! As preposterous and terrifying as this is — a whole society not only in jail but solitary confinement — it sums up the whole of Mecher’s view of disease. It’s also completely wrong.

Pathogens are part of our world; they are generated by human contact. We pass them onto each other as the price for civilization, but we also evolved immune systems to deal with them. That’s 9th-grade biology, but Mecher didn’t have a clue.

Fanatics Win the Day

Jump forward to March 12, 2020. Who exercised the major influence over the decision to close schools, even though it was known at that time that SARS-CoV-2 posed almost risk to people under the age of 20? There was even evidence that they did not spread COVID-19 to adults in any serious way.

Didn’t matter. Mecher’s models — developed with Glass and others — kept spitting out a conclusion that shutting down schools would drop virus transmission by 80%. I’ve read his memos from this period — some of them still not public — and what you observe is not science but ideological fanaticism in play.

Based on the timestamp and length of the emails, he was clearly not sleeping much. Essentially he was Lenin on the eve of the Bolshevik Revolution. How did he get his way?

There were three key elements: public fear, media and expert acquiescence, and the baked-in reality that school closures had been part of “pandemic planning” for the better part of 15 years. Essentially, the lockdowners, over the course of 15 years, had worn out the opposition. Lavish funding, attrition of wisdom within public health, and ideological fanaticism prevailed.

Figuring out how our expectations for normal life were so violently foiled, how our happy lives were brutally crushed, will consume serious intellectuals for many years. But at least we now have a first draft of history.

As with almost every revolution in history, a small minority of crazy people with a cause prevailed over the humane rationality of multitudes. When people catch on, the fires of vengeance will burn very hot.

The task now is to rebuild a civilized life that is no longer so fragile as to allow insane people to lay waste to all that humanity has worked so hard to build.

Tyler Durden Fri, 06/11/2021 - 21:40

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Brandon Smith: The Real Reasons Why California Leftists Are Terrified Of The AR-15

Brandon Smith: The Real Reasons Why California Leftists Are Terrified Of The AR-15

Authored by Brandon Smith via Alt-Market.us,

This past week a US District judge in California struck down the state’s 30 year ban on high capacity semi-automa

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Brandon Smith: The Real Reasons Why California Leftists Are Terrified Of The AR-15

Authored by Brandon Smith via Alt-Market.us,

This past week a US District judge in California struck down the state’s 30 year ban on high capacity semi-automatic rifles which leftists label “assault weapons”. The judge called the ban unconstitutional (which it is). In response, the progressive media has lost their collective minds, screeching in horror at the idea of AR-15 rifles being legal within the borders of their carefully manicured socialist Utopia state. Their most commonly expressed reaction seems to be fear.

Fear is rarely a rational thing. When someone operates based on fear they tend to make terrible decisions and support oppressive causes and laws. Fear leads to an obsession with control. Fearful people also tend to look for large mobs of other terrified people so they can feel safe and secure and anonymous. They want to be able to act impulsively on their fears without having to face consequences for it later.

Leftists are driven primarily by two factors: Narcissism, and yes, fear. I’ve discussed their narcissism at great length in past articles; now I think we should delve into their fear.

The most common leftist retort to the question “Why are you so afraid of the AR-15?” will usually be a snort of indignant disbelief followed by the words:

“Because it’s a military weapon designed to kill a lot of people quickly…idiot!”

But this is not an argument, it is an expression of irrational fear. Why are they, as individuals, afraid of the AR-15? What are the chances that they will EVER be faced with a person intent on killing them with an AR-15? And, why do they believe that disarming innocent law abiding Americans will somehow save them from their paranoia?

Let’s examine the first issue of statistical probability; how many people are actually killed by AR-15s each year? Not many according to the FBI, which does not track the stats on specific rifles, but does track the stats on all rifles together. And, as it turns out, only around 6% of all gun deaths involve rifles in the US each year.

How much of that 6% involves the use of military grade rifles like the AR-15? It’s impossible to say, but even if it was half, or 3% of all gun related crimes, that would still mean you have FAR more of a chance of being murdered by a knife or blunt object than an AR. By extension, Rifles overall are dwarfed by handgun murders, so, again, why are leftists so afraid of the AR-15?

What about mass shootings? It seems like the AR-15 is a favorite among mass shooters because of it’s “efficiency”, so is this reason enough to be fearful? According to the New York Times own analysis, the AR-15 was used to kill 173 people in mass shootings in the US from 2007 to 2017. Meaning around 17 homicides per year over a decade can be attributed to the rifle. Again, the AR is dwarfed by almost all other weapons in homicide including knives, even when accounting for mass shootings.

With the sheer number of military grade weapons in the hands of civilians in the US there should be mass homicides everywhere you look if you take the common position of the typical progressive gun grabber. But, this is not the case. In fact, if you want to increase your chances of being killed by a gun, move to a major Democrat run city like Chicago, New York or Philadelphia. In Chicago, there were 4033 shootings and 784 homicides, predominantly in black neighborhoods and primarily with handguns.

So, statistically, access to AR-15s does not increase gun homicides. But what about living in a black neighborhood in leftist run Chicago under some of the strictest gun laws in the country? Yes, your chances of being shot are MUCH higher (just not by an AR-15).

Since the math does not add up in favor of the leftists, perhaps we should examine other factors that might be driving them to focus on the AR in particular. Let’s talk about “precedence”…

Look at it this way – States like California are a petri dish, a testing ground for the future that leftists want for the entire country. There is an old saying that “As goes California, so goes the US”, and this is because California is often where most experimental legislation is pushed; legislation that violates the boundaries of what the constitution allows. Sometimes it’s New York or New Jersey or some other blue state, but most of the time CA is where unconstitutional precedents are set. Its massive population and large number of electoral votes make it a perfect target for conditioning the wider public to further restrictions on their freedoms.

This explains some of the fear the media is showing regarding the latest federal court decision on military grade weapons like the AR. Political elites see California as their own little kingdom with their own special laws, and they plan to eventually spread those laws across America using California as the model. But, if such laws are overturned as unconstitutional, then the precedent actually works in reverse. Now, the leftists are concerned that an overturned gun ban in CA means more blue states will follow and their entire gun grabbing scheme will go out the window.

The leftist mind thinks in terms of unchecked and unhinged “democracy”. Meaning, they believe that the majority is paramount; the majority is law. If a majority in a society wants to take away your freedoms, then they have the right to because they have the mob on their side. 51% rules over the lives of the other 49%. But this is not how things work in a Constitutional Republic.

Under the Bill of Rights your freedoms are codified and sacrosanct. They are inherent and gifted by God (or whatever you happen to believe in); government has no domain over these rights. The right to firearms and self defense is one of these inherent qualities. It does not matter what the State of California thinks, or even what the “majority” of people in California think. If an American in California wants to own an AR-15, then he/she has the right to own an AR-15.

We also cannot ignore the fact that leftists have an insatiable appetite for collectivism, usually in the name of the “greater good”. Collectivism is basically totalitarianism disguised as humanitarianism. They know what’s best for you, and they are going to make sure you follow THEIR plan for your life.

The AR-15 is indeed a weapon in military use, and maybe this is what frightens leftists the most. Not because they are personally more likely to be shot by one (we’ve already proven that notion false), but because leftists desire control over all else, and with military grade weapons in the hands of the public control becomes much more difficult. ALL totalitarian governments seek to first disarm the people they intend to enslave or destroy. This is a fact.

When a group of people in power are working hard to remove defensive or even offensive weapons from your hands, it’s best to assume that their intentions are malevolent. They are not trying to help you, they are trying to help themselves.

They will deny this motive to the grave, but look at how the political left has been acting lately: They are the only people that have supported mass censorship of opposing viewpoints. They are the only people that are supported by international conglomerates and Big Tech companies. They are the only people that supported the pandemic lockdowns, which were completely useless in stopping the spread of covid, but they were very useful in killing hundreds of thousands of small businesses across the US. They are also the only people in favor of vaccine passports which would destroy the very fabric of our society and erase what is left of our freedoms.

It’s not really surprising that they want to disarm us as well.

Of course, they will claim that this argument is “silly”. After all, what can an AR-15 do against an Apache helicopter or a Abrams battle tank? Well, these rifles in the right hands can do a hell of a lot to stop a technologically advanced military, as we have seen for the past two decades in Afghanistan. Let us not play games; there is a reason why leftists and elites are obsessed with our disarmament. If military grade rifles were not a threat to them, then they would not be going after them so aggressively.

Finally, the mainstream media has rolled out all the typical propaganda tools when it comes to spinning the federal decision in CA, including attacking the judge and his character. Almost every single article on this issue focuses on the fact that the judge compared the AR-15 to a “Swiss Army knife”.

The left will continue to use this narrative as a means to distract from the real problem at hand because false conflations and straw man arguments have worked for them in the past. Clearly, the judge was not trying to say that an AR-15 and a Swiss Army knife are exactly the same, or that they are equally capable of killing people. The logical interpretation is that the AR-15 is a tool like any other tool, and it has multiple uses. It is a utilitarian object, not an inherently demonic death machine as leftists would have us believe.

Gun grabbers love to make the argument that firearms are only designed for one purpose: “Killing”. This is a lie. They are also tools for self defense. They are a means to defuse a violent situation before it even happens. There are thousands of videos on the web showing people with criminal intent running away from a Good Samaritan with a gun. There is no way of telling how many potential victims have been saved by the mere presence of a firearm, but the accounts are documented and numerous.

This is on top of all the other uses for guns, including hunting and sporting uses. So, yes, the judge is absolutely correct; an AR-15 is a multipurpose tool, just like a Swiss Army knife.

In my view, the gun control lobby in America is in the midst of a considerable decline, and maybe it is even about to die. The political left has long operated on the mantra that “the squeaky wheel gets the oil”. In other words, they think if they whine long enough and loud enough about an issue someone will come along and give them what they want just to shut them up, even if what they want is illogical or morally bankrupt.

This strategy has worked out for them for many decades so it’s not surprising that they keep using it, but times are changing. Now, the squeaky wheel gets no oil, at least not from gun owners. The squeaky wheel gets nothing.

Gun control is the big line in the sand for most law abiding conservatives and moderates, and we have grown tired of the debate because it’s no longer a debate, it’s a imposition of ideology and cultism. All the facts are on the side of gun owners. All the legal protections are on the side of gun owners. All the moral dynamics are on the side of gun owners. As long as we stand our ground, there is nothing that leftists can do about it.

They can continue to lie, they can continue to threaten and they can continue exploiting emotional arguments, but they’ll NEVER get the guns. And, as we have seen recently, we might even start returning some of those gun rights and rifles to states like California, where fear was used to cloud the public mind and people were conned into compliance.

What are California leftists and their comrades in other blue states really afraid of? They are afraid that their strategies are failing, that the public is getting wise to their games, that their incrementalism only works for so long, that their true intentions have become transparent, that their narcissism has blinded them to their own frailties, that the law is not their plaything and that every piece of constitutional ground they have stolen over the decades could be taken back from them in the blink of an eye; as fast as a speeding bullet.

Leftists and totalitarians fear the AR-15, but what they fear more is what it represents. And with each carefully placed practice shot at every gun range across America, they hear the crushing sound of inevitability.

*  *  *

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Tyler Durden Fri, 06/11/2021 - 22:20

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“Diamonds in the Rough” – Last Week’s Darlings and Dud

Each week in my DecisionPoint Diamonds reports, I give readers 10+ stock picks to consider. This week, we had 11 picks. I look for stocks that are beginning to show positive momentum, bullish chart patterns, breakouts and improving relative strength among

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Each week in my DecisionPoint Diamonds reports, I give readers 10+ stock picks to consider. This week, we had 11 picks. I look for stocks that are beginning to show positive momentum, bullish chart patterns, breakouts and improving relative strength among not only the SPX, but also against its industry group.

Today, I thought I would share three of last week's "Diamonds in the Rough." I have two "Darlings" and one "Dud." They aren't all winners and certainly are not "sure things," so I feel it is appropriate to show you the trade that didn't work out.

This week's "Darlings" come from the Biotech industry group, which really broke out this week. This week's "Dud" wasn't actually too bad. It was a timing issue, but it actually looks pretty good going forward. As part of a DP Diamonds subscription, you also get entrance into the Friday "Diamond Mine" where we review in detail the "Diamonds in the Rough" that presented for the week. I also take symbol requests and we look at BUY points for symbols with promise. An added bonus is that, at the end, we "mine" for new opportunities going into next week.

Below I have the "Diamonds in the Rough" as they were presented originally, followed by my comments on the chart today.

Darling #1:

Illumina, Inc. (ILMN) - Up +4.43% since 6/9


EARNINGS: 8/5/2021 (AMC)


Illumina, Inc. engages in the development, manufacturing, and marketing of life science tools and integrated systems for large-scale analysis of genetic variation and function. It operates through Core Illumina segment, which serves customers in the research, clinical and applied markets, and enables the adoption of a variety of genomic solutions. The firm's products include instruments, kits and reagents, selection tools, software and analysis. Its services include sequencing and microarray services; proactive instrument monitoring; and instrument services, training and consulting. The company was founded by David R. Walt, John R. Stuelpnagel, Anthony W. Czarnik, Lawrence A. Bock and Mark S. Chee in April 1998 and is headquartered in San Diego, CA.

Below is the chart and commentary from Wednesday (6/9):

"ILMN is down -0.01% in after-hours trading. I was surprised I hadn't covered this one before. It's not as volatile as many of the biotechs out there. We have a nice breakout from a trading range. The breakout occurred Monday and price has held above support today and yesterday. The RSI is on the overbought side, so we should be aware of that. The PMO is on an oversold BUY signal and has now reached positive territory. There was a recent IT Trend Model "Silver Cross" BUY signal when the 20-EMA crossed above the 50-EMA. The OBV is confirming the rally and the SCTR is almost in the "hot zone" above 75, meaning it is in the upper quartile among all large-cap stocks. Outperformance is clear. I like that you can set a reasonable 7% stop."

Here is today's chart:

The biggest flaw is the overbought RSI, which is flattening out. ILMN needs to consolidate Thursday's breakout. It began to today, I still love this Biotech and you can tighten the stop more too. The SCTR is now in the "hot zone" above 75, meaning it is in the top quartile among its peers in the large-cap "universe." Also, regarding the SCTR, the calculations are heavily based on intermediate- and long-term indicators.


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Darling #2:

Novavax, Inc. (NVAX) - Up +2.31% since yesterday


EARNINGS: 8/9/2021 (AMC)

Novavax, Inc. focuses on the discovery, development and commercialization of vaccines to prevent infectious diseases. It provides vaccines for COVID-19, seasonal flu, respiratory syncytial virus, Ebola and Middle East respiratory syndrome. The company was founded in 1987 and is headquartered in Gaithersburg, MD.

Here is the chart and commentary from Thursday (6/10):

"NVAX is up +0.16% in after hours trading. I covered NVAX as a Reader Request on July 30th, 2020. It hit its 9.2% stop when price dropped in August, so the position would be closed at this point. However, I like it today given the improvement in the Biotech space and knowing that this one is a leader based on the 98.9 SCTR. Today, price pulled back toward the breakout point. This took the RSI away from overbought territory. The PMO shows no damage and is now rising in positive territory on an oversold crossover BUY signal. Volume is coming in and, despite the pullback, the OBV didn't sustain much damage either. The stop is set below the August 2020 top."

Below is today's chart & commentary:

The trade still looks good and it isn't too late for entry. The RSI is positive and not overbought. The PMO is rising strongly on a BUY signal. Most interesting is the nearing of an IT Trend Model "Silver Cross" BUY signal, which will be triggered when the 20-EMA crossover above the 50-EMA. Join me in the free Trading Room on Monday and we'll explore the best BUY points! Register HERE if you haven't already.



Dud:


Dycom Industries, Inc. (DY) - Down -2.91% Since 6/8


EARNINGS: 8/25/2021 (BMO)


Dycom Industries, Inc. provides contracting services throughout the United States. Its services include engineering, construction, maintenance and installation services to telecommunications providers, underground facility locating services to various utilities, including other construction and maintenance services to electric and gas utilities, and others. The company was founded in 1969 and is headquartered in Palm Beach Gardens, FL.

Below is the chart and commentary from Tuesday (6/8):

"DY is up +0.72% in after hours trading. I covered DY in the October 14th 2020 Diamonds Report. I didn't put a stop on the chart, but an 8% stop wouldn't have triggered even on the big gap down in November. This means that the position is up +26.7%. The end of May was killer for DY, but it is retracing the decline rapidly. The first of two gaps has now been covered. Today's rally pushed DY above the 20-EMA. The PMO had already begun to curl upward. The RSI isn't positive yet, but it is on its way. The industry group is outperforming the SPX and DY is outperforming both. The stop is set below the gap, right around the 200-EMA at $77.28."

Below is today's chart:

While a lot of things went wrong on this chart, everything still looks bullish, especially after today's strong rally. A short-term flag has formed. The PMO had a positive crossover today. The RSI is still negative, but it is rising again. The timing was just off for it to look good this week. However, if we revisit this chart later, I expect that flag will have resolved upward and we'll be challenging the highs from April and May.

 Happy Charting! - Erin


Technical Analysis is a windsock, not a crystal ball.


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